Figure 6From: Phosphorylation of HIV Tat by PKR increases interaction with TAR RNA and enhances transcriptionTransactivation of the HIV-1 LTR by HIV-1 Tat wild-type and mutants. Duplicate wells of confluent HeLa cells were transfected for 6 h with pcDNA3.2-DEST-Tat, pHIV-LTR-CAT and β-actin luciferase. Cells were harvested 24 h post transfection and assayed for CAT activity, luciferase activity and protein concentration. The graph shows the results of three separate experiments.Back to article page