Figure 4

Inhibition of HCMV by IFN-α, IFN-β and/or IFN-γ is not a result of decreased viral entry into cells. Ethidium bromide-stained IE exon 4 PCR products amplified from HCMV-infected HFFs pre-treated with either vehicle (A) or 100 IU/ml of IFN-α (B), IFN-β (C), IFN-γ (D), IFN-α and IFN-γ (E) or IFN-β and IFN-γ (F). From left to right, PCR products were amplified from H₂O control, 100 ng of uninfected (UI) HFF DNA or 100 ng of HCMV-infected HFF DNA harvested from cells inoculated for 2 h at MOIs of 0.3 to 30. GAPDH PCR products were run along side IE exon 4 PCR products and served as internal loading controls (data not shown).