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Figure 1 | Virology Journal

Figure 1

From: Recombinant Tula hantavirus shows reduced fitness but is able to survive in the presence of a parental virus: analysis of consecutive passages in a cell culture

Figure 1

Checking of specificity of RT-PCRs for the wt and the recombinant S RNA segments. Lines 1–3: products of RT-PCR with primers VF738 and VR855 on RNA from cells infected with TULV02 (line 1), on RNA from cells infected with the recTULV (line 2) and on the mechanical mixture of both RNA preparations (line 3). Lines 5–7: the corresponding products of RT-PCR with primers RECF738 and RECR855. Lines 4 and 8 show negative controls. M, molecular weight marker; bands of 147 and 110 bp are indicated by arrows.

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