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Figure 2 | Virology Journal

Figure 2

From: Simian immunodeficiency virus (SIV) envelope quasispecies transmission and evolution in infant rhesus macaques after oral challenge with uncloned SIVmac251: increased diversity is associated with neutralizing antibodies and improved survival in previously immunized animals

Figure 2

Characterization of the dominant variants in SIVmac251-5/98 virus stock. HMA analysis of all four endpoint variants shown in Fig. 1 (lanes 1–4) and all possible pairwise mixtures of those variants (lanes 5-10) are shown. Letters B, C, and F refer to the dilution series shown in Fig. 1. Lane numbers refer to the lane designations of the variants that were mixed in lanes 5–10 (e.g., L1 + L2 indicates that the variants shown in lanes 1 and 2 were mixed). Lane 6 shows that the 2 endpoint variants labeled VSEV-1 (B 10-8 and C 10-7) are similar variants due to the formation of a single homoduplex and no heteroduplexes when these 2 variants were mixed. Lane 9 indicates that the 2 endpoint variants labeled VSEV-2 (C 10-6 and F 10-6) in Fig. 1 are very similar. The formation of heteroduplexes and two main homoduplexes in the mixtures shown in lanes 5, 7, 8, and 10 indicate that VSEV-1 and VSEV-2 do not share the same V1–V2 envelope sequence.

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Virology Journal

ISSN: 1743-422X