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Figure 1 | Virology Journal

Figure 1

From: Simian immunodeficiency virus (SIV) envelope quasispecies transmission and evolution in infant rhesus macaques after oral challenge with uncloned SIVmac251: increased diversity is associated with neutralizing antibodies and improved survival in previously immunized animals

Figure 1

Characterization of variants in SIVmac251-5/98 virus stock. HMA analysis of four separate dilution series of viral RNA from the SIVmac251-5/98 virus stock is shown. The presence of multiple bands in the undiluted samples (lane 1 of each gel) reveals the virus stock was comprised of a diverse viral population. The last lane of each gel shows the variants in the highest dilution that yielded an RT-PCR product. Dilution series A shows an example of a dilution experiment that did not result in a virus stock endpoint (homogenous variant population); the 10-6 dilution included more than 1 variant, while the next dilutions (10-7–10-9) dilution did not yield RT-PCR products, and therefore no variant pattern is shown for those dilutions. This dilution pattern was observed in 3 of 6 dilution series (other 2 not shown). For the other 3 dilution series (B, C, and F), the variant (band) remaining in the highest dilution was considered to be the most common variant, and was designated the Virus Stock Endpoint Variant (VSEV). Dilution series B: no product was amplified from the 10-7 dilution (lane 8), but a product was amplified from the 10-8 dilution (lane 9). Dilution series C: lanes 7 and 8 show the presence of 2 different variants (VSEV-1 and VSEV-2) in the endpoint dilutions (10-6 and 10-7) of this series. Dilution series F; the 10-6 dilution in this series harbored an endpoint variant that migrated to the same gel position as VSEV-2 in dilution series C.

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