The infection ability of BRV was partially recovered with the replenishment of exogenous cholesterol. MA-104 cells were pretreated with 10 mM MβCD for 30 min, and then supplied with 1.5 mg/ml exogenous cholesterol for 1 h followed BRV infection. (A) Content of cellular cholesterol was determined with Amplex Red cholesterol assay kit. (B) 12 h post infection, virus titers were determined. Each experiment was performed in triplicate. “***” denotes extremely significant difference in statistics (P < 0.001).