Figure 4
From: Mesoniviruses are mosquito-specific viruses with extensive geographic distribution and host range
Polypeptides encoded in the ORF3a/ORF3b region of mesonivirus genomes. (A). Clustal X alignment of the sequences of polypeptides encoded in ORF3a illustrating the predicted signal peptide, exodomain, transmembrane domain and cytoplasmic domain, two domains of highly conserved sequence (boxed), conserved cysteine residues (black shading) and a potential N-glycosylation site (underlined). (B). Clustal X alignment of the sequences of polypeptides encoded in ORF3b illustrating the cytoplasmic domain, transmembrane domain and ectodomain and a potential N-glycosylation site (underlined). (C). Clustal X alignment of the sequences of polypeptides that would be generated by -1 ribosomal frame-shift at the predicted ‘slippery’ sequence (CACUUUU) in the overlap region. (D). Schematic representation of the predicted membrane topology of the putative 3a, 3b and 3ab glycoproteins using SignalP and TMHMM and NetNGlyc (http://www.expasy.org).