Skip to main content
Figure 3 | Virology Journal

Figure 3

From: Cloning and expression of N22 region of Torque Teno virus (TTV) genome and use of peptide in developing immunoassay for TTV antibodies

Figure 3

Solubility and purification of expressed protein. A). Coomassie stained 12% SDS-PAGE of soluble and pellet fractions. Bold arrow denotes 25 kDa expression product. Lane L: Protein marker; Lane S: Protein from soluble fraction; Lane P: Protein from pellet/insoluble fraction. B). Coomassie stained 12% SDS-PAGE of fractions obtained during Ni-NTA column purification. Bold arrow denotes 25 kDa expression product. Single bands were obtained in elution fractions. Lane L: Protein marker; Lane CL: Cleared lysate; Lane FT: Flow-through fraction; Lane W1: Wash I fraction; Lane W2: Wash II fraction; Lane E1: Elution I fraction; Lane E2: Elution II fraction.

Back to article page

Virology Journal

ISSN: 1743-422X