Samba virus genome (circular) and phylogeny: (A) Estimated Samba virus genome size assembled using the Perl-based program ABACAS 1.3.1-1. The predicted ORFs are highlighted in blue, revealing a very low proportion of noncoding DNA. The innermost circle indicates the G-C content. Regions with a G-C content higher than the average (28%) are highlighted in green, and lower than average in purple. (B) Phylogenetic tree (neighbor joining, 1,000 bootstrap replicates, n. differences model) based on the ribonucleotide reductase predicted amino acid from the Samba virus and other nucleocytoplasmic large DNA viruses. The Samba virus clusters with members of Megavirales group A, which is comprised of Mimivirus and Mamavirus. The tree is unrooted. The values near the branches are bootstrap values calculated using the MEGA 4 program and are used as confidence values for the tree branches.