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Figure 1 | Virology Journal

Figure 1

From: Difference in cytokine production and cell cycle progression induced by Epstein-Barr virus Lmp1 deletion variants in Kmh2, a Hodgkin lymphoma cell line

Figure 1

Characterization of the KMH2-pRT-LMP1 established cell lines. (a) Linear representation of WT-LMP1 and the 10 and 23 amino-acid deletions corresponding to the del30 and del69-LMP1 variants. (b) RT-PCR or (c) Western blot of EBNA1 and LMP1 in non-induced cells and in doxycyclin-induced cells expressing WT-LMP1, del30-LMP1 and del69-LMP1 variants. 293-HEK cells transiently transfected with pcDNA-WT-LMP1 were used as positive control for immunoblotting. (d) Comparison of LMP1 expression by flow-cytometry in the KMH2-pRT-LMP1 cells. Doxycyclin-induced cells are represented in green and non-induced cells in black. Data are representative of 3 experiments.

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Virology Journal

ISSN: 1743-422X