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Figure 4 | Virology Journal

Figure 4

From: Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer

Figure 4

Characteristics of recombinant NDV in lung cancer cell line A549. (A) Growth dynamics of the recombinant viruses in A549 cells. The A549 cells were infected with rNDV-D90, rNDV-GFP or D90 strain at a MOI of 0.01. Every 12 h post-infection, the cells were harvested. Virus titers at each time point were determined by TCID50 titration in A549 cells. (B) Effect of rNDV-D90 or rNDV-GFP on cell viability was assessed in A549 cells by MTT assay. Data are presented as means (±SD) derived from six independent measurements. (C) DAPI staining. A549 cells were infected with rNDV-D90 or rNDV-GFP for 16 h at a MOI of 0.1. Nuclei are stained blue. The cells infected with viruses show the characteristics by chromatin condensation with brighter fluorescence.

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