Table 1 Sequences of primers and probes used in real-time qPCR assay and cDNA synthesis
From: Antibody-dependent infection of human macrophages by severe acute respiratory syndrome coronavirus
Gene | Sequences | Amplification product (bp) | GeneBank accession number |
|---|---|---|---|
18S rRNA | (F) 5′-CggAggTTCgAAgACgATCA | 90 | NR_003286 |
(R) 5′-ggCgggTCATgggAATAAC | |||
(P) 5′(HEX) ATACCgTCgTAgTTCCgACCA (BHQ) | |||
Nucleocapsid | (F) 5′-ACCAgAATggAggACgCAATg | 85 | AY278491 |
(R) 5′-gCTgTgAACCAAgACgCAgTATTAT | |||
(P) 5′(FAM) ACCCCAAggTTTACCC (NFQ) | |||
ORF1b | (F) 5′-CAgAACgCTgTAgCTTCAAAAATCT | 68 | AY278491 |
(R) 5′-TCAgAACCCTgTgATgAATCAACAg | |||
(P) 5′(FAM) TCTgCGTAggCAATCC (NFQ) |