Figure 5

Lethal EV71 challenge of newborns from immunized female mice. Two-day-old pups from immunized female mice were intracerebrally inoculated with EV71. (A) Unchallenged suckling mouse (B) representative mouse in the PBS group with limb paralysis and wasting (arrows) at 3 days post-infection with EV71 virus; (C) protected mouse challenged with EV71 virus; (D) survival rate after viral challenge of suckling mice from mothers immunized with different oral vaccines; (E) mean clinical scores for clinical symptoms of pups from female mice immunized with different vaccines; (F) weight changes in suckling ICR mice after EV71 challenge. Body weights were recorded for all surviving mice daily until 14 days post-infection.