Serological characterization of recombinant mTLNE. (A) Western blotting assay using mouse polyclonal antibody against EV71. Lanes 1: mTLNE proteins; Lanes 2: Trx proteins. (B) ELISA using rabbit and mouse polyclonal antibodies against EV71. Recombinant mTLNE could be specifically recognized by the corresponding mouse and rabbit antibodies compared with the control Trx. The cut-off value for the ELISA is shown by a dotted line.