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Figure 3 | Virology Journal

Figure 3

From: Membrane-bound IL-12 and IL-23 serve as potent mucosal adjuvants when co-presented on whole inactivated influenza vaccines

Figure 3

CYT-IVAC~IL12and CYT-IVAC~IL23boost mucosal anti-viral responses. (A) Mice were vaccinated with either non-adjuvanted WIV or CYT-IVACs. Blood was collected post-boost on day 35 and serum antibody titers for influenza-specific IgA were determined by ELISA. (B) Mice were challenged with 100LD50 dose of mouse adapted A/PR/8/34 on day 36 following vaccination. At day 4 following challenge, animals (n = 5 mice/group) were sacrificed, nasal washes were collected in PBS (2 ml total volume) and flash frozen. Levels of influenza-specific IgA were quantitated by ELISA, with IgA standard curve extrapolation. Data represents mean concentration ± SE. (A) IM Group *p < 0.05 compared to WIV; IN Group *p < 0.05, ***p < 0.001 compared to WIV by One way ANOVA, (B) ***p < 0.001 compared to WIV, One way ANOVA Bonferroni’s multiple comparison test).

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