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Figure 1 | Virology Journal

Figure 1

From: Membrane-bound IL-12 and IL-23 serve as potent mucosal adjuvants when co-presented on whole inactivated influenza vaccines

Figure 1

Western blot analysis of CYT-IVAC~IL12and CYT-IVAC~IL23. Whole viral lysates were run on 12% SDS-PAGE gel, blotted on PVDF membrane and incubated with IL-12/23p40 antibody followed by anti-species secondary antibodies conjugated to HRP. (A) Dilutions of CYT-IVAC~IL-12 ranging from 10 μg to 0.5 μg of protein and (B) CYT-IVAC~IL-23 (lane 2) (5 μg) and PR8 (lane 1) (5 μg) were probed with anti-IL12/23 p40 antibody (eBioscience). (C) Quantitation of virus-incorporated cytokine (pg of cytokine per μg of vaccine) (FlowCytomix™, eBioscience).

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Virology Journal

ISSN: 1743-422X