Skip to main content

Table 2 Oligonucleotide primers used for RT-LAMP assay

From: A loop-mediated isothermal amplification assay for the visual detection of duck circovirus

Primer name

Sequence (5′–3′)

Genome position

FIP = F1c + F2

TTCAGGAATCCCTGAAGGTG-ATCGTCGGMGAGGAVAAGG

F1c,203–222, F2,167–185

BIP = B1c + B2

GCGMGAGCTGCCGCCCT-TCTTCVTCAGATCCCCGG

B1c,236–252, B2,292–309

F3

AGTTBTGCACGCTCGACAAT

135–154

B3

GTCGACTCTTTGGMGCAATA

320–339

LoopF

AGGYGTVCCVTTCGCGC

186–202

LoopB

AGGAAGAGCCTGGCTCTC

268–285

  1. Genome position according to the DuCV complete genome sequence (GenBank accession no. DQ100076).
  2. Abbreviations are as follows: M = A or C; V = A or C or G; B = T or G or C.
Back to article page

Virology Journal

ISSN: 1743-422X