Figure 5From: The YXXΦ motif within the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein is crucial for its intracellular transportIncreased lipid droplet targeting is associated with increased lysosomal degradation. (A) Expression levels of the wild type and mutant 3a proteins were evaluated 48 hrs post-transfection into Huh7 cells by western blotting with anti-3a antibodies and semi-quantitative RT-PCR with gene-specific primers. (B) In vitro transcription-translation analysis of the wild type and mutant 3a proteins in a rabbit reticulocyte lysate system. (C) Colocalization of the ∆YXXΦ mutant protein and the late endosome/lysosomal marker Rab7. (D) Transfected Huh7 cells expressing the ∆YXXΦ mutant protein were treated with Brefeldin-A or Aprotinin for 12 hr before harvest. Western blotting was done with anti-3a and ERK (loading control) antibodies. Quantitation was done using Image-J software. Data shown are representative of two different experiments.Back to article page