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Figure 5 | Virology Journal

Figure 5

From: The YXXΦ motif within the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein is crucial for its intracellular transport

Figure 5

Increased lipid droplet targeting is associated with increased lysosomal degradation. (A) Expression levels of the wild type and mutant 3a proteins were evaluated 48 hrs post-transfection into Huh7 cells by western blotting with anti-3a antibodies and semi-quantitative RT-PCR with gene-specific primers. (B) In vitro transcription-translation analysis of the wild type and mutant 3a proteins in a rabbit reticulocyte lysate system. (C) Colocalization of the ∆YXXΦ mutant protein and the late endosome/lysosomal marker Rab7. (D) Transfected Huh7 cells expressing the ∆YXXΦ mutant protein were treated with Brefeldin-A or Aprotinin for 12 hr before harvest. Western blotting was done with anti-3a and ERK (loading control) antibodies. Quantitation was done using Image-J software. Data shown are representative of two different experiments.

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