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Table 1 DNA constructs and administration schemes used in immunization studies

From: H5-based DNA constructs derived from selected highly pathogenic H5N1 avian influenza virus induce high levels of humoral antibodies in Muscovy ducks against low pathogenic viruses

Group (number of ducks) Treatment(a)/origin/clade-genotype(b)of the HA gene for DNA immunization Accession number(c)and reference
H5N2 LP (15) N (LP H5N2)/A/duck/France/05057b/2005 AJ972673 [20]
Opt 2.1 (30) Opt mut (HP H5N1)/A/chicken/Indonesia/7/03/2.1 ABO30346
2.2.1 G1 (15) N mut (HP H5N1)/A/common pochard/France/06167/06/2.2.1 G1 AM498628 [22]
2.2.1 G2 (15) N mut (HP H5N1)/A/swan/France/06299/06/2.2.1 G2(d) EF395820 [22]
Opt 2.2.1 (15) Opt mut (HP H5N1)/A/turkey/Turkey/1/05/2.2.1 ABD73284
Opt 2.3.4 (15) Opt mut (HP H5N1)/A/duck/Laos/3295/06/2.3.4 ACH68553
Negative control (15) No HA gene(e) -
  1. Prime immunization was done at five weeks and boost at eight weeks of age with 100 μg DNA per duck in all groups. Bleeding was done three weeks after the boost.
  2. The percentages of amino acid identity and the corresponding differences between all six H5 sequences are displayed in Additional file 1: Tables S2 and S3.
  3. (a)N = native; opt = chicken codon optimized; mut = mutated cleavage site HP → LP.
  4. (b)When relevant.
  5. (c)Genbank.
  6. (d)The most represented subgroup in 2006 French outbreaks.
  7. (e)Empty pcDNA3.1(-).