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Table 1 LAMP primers for detection of PKV

From: Rapid detection of porcine kobuvirus in feces by reverse transcription loop-mediated isothermal amplification

Primer name

Length

Type

Genome position

Sequence (5'-3')

F3

18

Forward outer

7525-7342

CCCGCTACATCGAGACCA

B3

20

Reverse outer

7530-7549

TGGGTGGATCACACCCATAG

FIP

42

Forward inner

F1:7395-7416

GCCAACACATCCAGACGGGTTA-

F2:7350-7369

CTCACGCCATGTCTTTGGTA

BIP

42

Reverse inner

B1:7450-7471

TGTGTCCTCTCCGCTCTGATCC-

 

B2:7510-7529

ATCACATCATCACCGTAGGC

  1. The position of LAMP primers are shown relative to the CH/HZ/2011 strain PKV (Accession No. JX827598).