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Figure 2 | Virology Journal

Figure 2

From: Rapid detection of porcine kobuvirus in feces by reverse transcription loop-mediated isothermal amplification

Figure 2

Optimal temperature and time for the PKV RT-LAMP assay. The optimization of the PKV RT-LAMP was analyzed by performing the reaction at 62, 63, 64 or 65°C for 1 h. The reaction results are shown in lanes 1–4, respectively (A). The same reaction was performed at 64°C for 30, 40, 50, or 60 min. The RT-LAMP results are shown in lanes 1–4, respectively (B). Lane M: DNA marker; lane 5: Negative control.

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Virology Journal

ISSN: 1743-422X