Skip to main content

Table 4 Scheme of immunization of animals with recombinant influenza A viruses of the subtypes Н5N1 and H1N1

From: Influenza viral vectors expressing the Brucella OMP16 or L7/L12 proteins as vaccines against B. abortus infection

Species Viral construct Route of administration Number of animals Prime vaccination dose (Н5N1), Log10EID50/animal Boost vaccination dose (Н1N1), Log10EID50/animal
Mice Monovalent Flu-NS1-124-L7/L12 i.n. 10 5.97 6.39
c. 10 5.58 6.08
s.c. 10 6.58 7.00
Monovalent Flu-NS1-124-Omp16 i.n. 10 5.51 5.64
c. 10 5.20 5.33
s.c. 10 6.12 6.25
Bivalent vaccine formulation Flu-NS1-124-L7/L12 + Flu-NS1-124-Omp16 i.n. 10 5.58 + 5.20 6.08 + 5.33
c. 10 5.27 + 4.89 5.69 + 4.99
s.c. 10 6.27 + 5.81 6.69 + 5.94
Guinea pigs Monovalent Flu-NS1-124-L7/L12 i.n. 5 6.28 6.70
c. 5 5.89 6.39
s.c. 5 6.58 7.00
Monovalent Flu-NS1-124-Omp16 i.n. 5 5.82 5.95
c. 5 5.51 5.64
s.c. 5 6.12 6.25
Bivalent vaccine formulation Flu-NS1-124-L7/L12 + Flu-NS1-124-Omp16 i.n. 5 5.97 + 5.51 6.39 + 5.64
c. 5 5.58 + 5.20 6.00 + 5.30
s.c. 5 6.27 + 5.81 6.69 + 5.94
  1. The amounts of inoculate for the intranasal (i.n.), conjunctival (c.) or subcutaneous (s.c.) immunization routes were 50, 25, and 200 μl for mice, and 100, 50 and 200 μl for guinea pigs, respectively.