Table 4 Scheme of immunization of animals with recombinant influenza A viruses of the subtypes Н5N1 and H1N1
Species | Viral construct | Route of administration | Number of animals | Prime vaccination dose (Н5N1), Log10EID50/animal | Boost vaccination dose (Н1N1), Log10EID50/animal |
|---|---|---|---|---|---|
Mice | Monovalent Flu-NS1-124-L7/L12 | i.n. | 10 | 5.97 | 6.39 |
c. | 10 | 5.58 | 6.08 | ||
s.c. | 10 | 6.58 | 7.00 | ||
Monovalent Flu-NS1-124-Omp16 | i.n. | 10 | 5.51 | 5.64 | |
c. | 10 | 5.20 | 5.33 | ||
s.c. | 10 | 6.12 | 6.25 | ||
Bivalent vaccine formulation Flu-NS1-124-L7/L12 + Flu-NS1-124-Omp16 | i.n. | 10 | 5.58 + 5.20 | 6.08 + 5.33 | |
c. | 10 | 5.27 + 4.89 | 5.69 + 4.99 | ||
s.c. | 10 | 6.27 + 5.81 | 6.69 + 5.94 | ||
Guinea pigs | Monovalent Flu-NS1-124-L7/L12 | i.n. | 5 | 6.28 | 6.70 |
c. | 5 | 5.89 | 6.39 | ||
s.c. | 5 | 6.58 | 7.00 | ||
Monovalent Flu-NS1-124-Omp16 | i.n. | 5 | 5.82 | 5.95 | |
c. | 5 | 5.51 | 5.64 | ||
s.c. | 5 | 6.12 | 6.25 | ||
Bivalent vaccine formulation Flu-NS1-124-L7/L12 + Flu-NS1-124-Omp16 | i.n. | 5 | 5.97 + 5.51 | 6.39 + 5.64 | |
c. | 5 | 5.58 + 5.20 | 6.00 + 5.30 | ||
s.c. | 5 | 6.27 + 5.81 | 6.69 + 5.94 |