Titers of antibodies against the L7/L12 and Omp16 proteins in mice from the experimental and control (on 28 [A] and 56 [B] days after a single vaccination with B. abortus 19 or administration of PBS) groups 28 days after prime (A) and boost (B) immunization by different routes of administration with mono- or bivalent vaccine formulations of the subtypes Н5N1 and Н1N1, as determined by ELISA. Data for the control group is not shown; i.n. – intranasally, c. – conjunctivally, s.c. – subcutaneously; Ag – antigen. The data are presented as geometric mean titer (GMT) ± standard error (SE); * P < 0.05, † P < 0.01 compared to single vaccination (n = 5 mice per group). Statistical analysis was performed using a one way ANOVA followed by Tukey’s multiple comparisons test.