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Table 1 Infectivity and latent period of pseudorecombinants formed between ToRMV-[BR:Ub1:96] and ToSRV-[BR:PG1:Pep:03] in tomato plants

From: Recombination and pseudorecombination driving the evolution of the begomoviruses Tomato severe rugose virus (ToSRV) and Tomato rugose mosaic virus (ToRMV): two recombinant DNA-A components sharing the same DNA-B

Combination

Latent period (days)a

Infected/Inoculated (%)b

  

ToRMV-A

ToRMV-B

ToSRV-A

ToSRV-B

ToYSV

ToRMV-A + B

14

11/12(92)

11/12(92)

-

-

-

ToSRV-A + B

14

-

-

13/13(100)

13/13(100)

-

ToRMV-A + ToSRV-B

14

13/13(100)

-

-

13/13(100)

-

ToRMV-B + ToSRV-A

14

-

12/13(92)

12/13(92)

-

-

ToRMV-A + B + ToSRV-A

14

12/13(92)

12/13(92)

1/13(8) c

-

-

ToRMV-A + B + ToSRV-B

14

10/13(77)

10/13(77)

-

0/13(0)

-

ToRMV-A + ToSRV-A + B

14

11/12(92)

-

1/12(8)

12/12(100)

-

ToRMV-B + ToSRV-A + B

14

-

12/13(92)

12/13(92)

4/13(31)

-

ToRMV-A + B + ToSRV-A + B

14

13/13(100)

13/13(100)

2/13(15)

1/13(8)

-

ToYSV-A + B

10

-

-

-

-

9/9(100)

  1. aNumber of days elapsed between inoculation and the appearance of symptoms.
  2. bNumber of infected plants/number of inoculated plants, verified by visual observation of symptoms and confirmed by RCA following digestion with component-specific restriction enzymes. The total number of inoculated plants represents two independent experiments.
  3. cValues in italics indicate the low detection of ToSRV components when inoculated simultaneously with ToRMV components.