Figure 1
From: Activation of the PKR/eIF2α signaling cascade inhibits replication of Newcastle disease virus
NDV infection induced the phosphorylation of PKR and eIF2α. HeLa cells were infected with NDV strain LaSota (A) or Herts33 (B) at an MOI of 1 and harvested at 6, 12, and 24 hpi, respectively. The cell lysates were collected and analyzed by western blot with anti-PKR, anti-p-PKR (T446), anti-eIF2α, and anti-p-eIF2α (S51) antibodies. β-actin was used as a protein loading control. HeLa cells were infected and harvested with different doses (0.5, 1, and 2 MOI) of NDV strain LaSota (C) or Herts33 (D) for 12 h. Proteins were analyzed by western blot analysis with anti-PKR, anti-p-PKR (T446), anti-eIF2α, and anti-p-eIF2α (S51) antibodies. β-actin was used as a protein loading control. E. Comparison of PKR and eIF2α phosphorylation levels in HeLa cells inoculated with replication-competent or UV-inactivated NDV strain Herts/33.