p65 expression in E. coli and western blotting. (A) Analysis recombinant p65 by SDS-PAGE. Lane 1, Molecular wt. marker; lane 2, uninduced E. Coli; lane 3, IPTG induced E. coli and lane 3, purified p65 (~65 kDa). (B) Immunoblot analysis of mid gut cells using anti-p65 antibody: (i) SDS-10% PAGE and (ii) western blot. Lane M, Molecular weight marker; lane 1, uninfected midgut; lane 2, infected midgut; lane 3, purified polyhedra; lane 4, virion particle; lane 5, purified recombinant p65. Arrow indicates position of p65.