EV71 uptake and replication. MDDCs or RD cells were incubated with EV71 for 1 h at 37°C, and then treated with trypsin to remove surface-bound viruses. Viral uptake and replication were quantified by detecting viral RNA level at the indicated time post-infection. (A, C) MOI 0.1, and (B) MOI 1. One representative from three donors with similar results is shown. Data are mean ± SD. ***P <0.001, **P <0.01, and *P <0.05 were considered significant differences in paired t test.