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Table 1 Description of nucleotide start/stop locations and amino acid length of ORFs in SSME, along with predicted functions

From: Complete genome analysis of a frog virus 3 (FV3) isolate and sequence comparison with isolates of differing levels of virulence

 

Start/stop (#aa)

 

ORF

SSME

Predicted function/conserved domains

1R

272-1042

Replication factor &/or DNA packing protein

255aa

2L

1649-2629

Myristylated membrane protein, DUF230 poxvirus protein, TM*

325aa

3R

3436-4752

IIV6 ORF 229L, SP

437aa

4R

4793-4975

TM

59aa

5R

5408-6022

FPV ORF 250, US22, herpes virus early nuclear protein

203aa

6R

6025-6252

SP*

74aa

7R

7043-7429

SP

127aa

8R

7521-11402

DNA-dependent RNA polymerase largest subunit

1292aa

9L

11771-14617

NTPase, SNF2 family, N-terminal, helicases C-terminal, DEAD/H helicases

947aa

10R

14633-15046

TM

136aa

11R

15396-15608

TM

69aa

12L

15674-16567

Unknown protein, SP

296aa

13R

17108-17314

SP

67aa

14R

17329-17688

Unknown protein, SP

118aa

15R

17784-18752

AAA-ATPase, poxvirus A32

321aa

16R

19032-19859

Integrase homologue

274aa

17L

20100-21608

SP

501aa

18 L

21645-21881

TM

77aa

19R

21933-24515

Similar to LCDV1 or f10L conserved uncharacterized protein, SP

859aa

20R

24562-25008

Unknown protein, TM

147aa

21L

25231-25890

ISKNV ORF 56L-like protein

218aa

22R

26020-28941

D5 family NTPase, ATPase

972aa

23R

29319-30467

SP

381aa

24Rº

30877-31947

SP

355aa

25R

32141-32929

P31K protein

261aa

26R

32996-33226

Truncated elf-2α homologue

75aa

27R

33757-36669

Tyrosine kinase, CAP 10, putative lypopolysaccharide modifying enzyme

969aa

28R

36718-37206

Unknown protein, SP

161aa

29L

37385-37681

SP

97aa

30R

37883-38035

TM

49aa

31R

38097-38516

SP

138aa

32R

38566-40458

Neurofilament triplet H1 protein

629aa

33R

40541-40732

Unknown protein, TM

62aa

34R

40875-41195

Human parainfluenza virus 1L like protein, TM

105aa

35L

41287-41748

SP

152aa

36L

41761-42384

SP

206aa

37R

42778-43413

NIF/NLI interacting factor

210aa

38R

43554-45251

Ribonucleoside diphosphate reductase alpha subunit barrel domain

564aa

39R

45357-45707

Hydrolase of the metallo-beta-lactamase superfamily

115aa

40R

45795-46343

TM

181aa

41R

46725-50222

RRV ORF-2-like protein, SP

1164aa

42L

50718-50975

SP

84aa

43R

50974-51491

TM

171aa

44R

51512-51697

SP

60aa

45L

51973-52383

LCDV1 ORF-88-like protein, SP

135aa

46L§

52437-53000

RGI 47L-like protein, SSTIV 049L-like protein, Neurofilament triplet H1-like protein, microneme/rhoptry antigen

186aa

47L

53125-53541

SP

137aa

48L

53544-53795

SP

82aa

49/50L¶

53904-55451

LCDV1 ORF 58-like protein, RGI ORF 50L-like protein, SSTIV ORF 052-like protein, SAP DNA binding domain

514aa

51R

55531-57216

Unknown, SP

560aa

52L

57473-58540

3-beta-hydroxy-delta 5-C27 steroid oxidoreductase-like protein, TM

354aa

53R

58878-60446

LCDV1 ORF-20-like protein, SP

521aa

54L

60677-60907

Nuclear calmodulin-binding protein

75aa

55L

60945-62240

Helicase-like protein

430aa

55R

61089-62228

FV3 40-kDa protein, SP

378aa

56R

62328-62765

SP

144aa

57R

62879-64375

Phosphotransferase, S-TKc, Serine/Threonine protein kinase

497aa

58R

64700-65413

SP

236aa

59L

65964-67022

RGV 9807 unknown protein, SP

351aa

60R

67184-70225

DNA polymerase-like protein, DNA polymerase family B exonuclease

1012aa

61L

70234-70416

SP

59aa

62L

70859-74524

DNA-directed RNA polymerase II second largest subunit RNA polymerase domain 6, 7, 3, 2 beta subunit

1220aa

63R

74903-75397

dUTPase-like protein

163aa

64R

75522-75809

Interleukin-1 beta convertase precursor, Caspase-recruitment domain/DEATH

94aa

65L#

N/A

SP

66L#

76106-76157

SP

16aa

67L

76212-77375

Ribonucleoside-reductase diphosphate beta subunit-like protein

386aa

68R

77657-77944

SP

94aa

69R

78080-78346

Unknown protein, TM

87aa

70R

78364-78738

SP

123aa

71R

78778-79011

SP

76aa

72L

79068-79784

SP

238aa

73L

80232-81206

NTPase/helicase-like protein

323aa

74L

81381-82493

SP

369aa

75L

82525-82779

LITAF/PIG7 possible membrane associated motif in LPS-induced tumor necrosis factor alpha factor, TM

83aa

76R

82842-83063

SP

72aa

77L

83060-83407

LCDV ORF 2-like protein, SP

114aa

78L

84007-84645

SP

211aa

79R

84781-86499

ATPase-dependent protease

571aa

80L

87122-88237

Ribonuclease III-like protein

370aa

81R

88293-88571

Transcription elongation Factor SII, C2C2 zinc finger

91aa

82R

88700-89173

Immediate-early protein ICP-18

156aa

83R

89623-90267

Cytosine DNA methyl-transferase

213aa

84R

90652-91389

LCDV 1-like proliferating cell nuclear antigen, SP

244aa

85R

91464-92051

Deoxynucleoside kinase

194aa

86L

92441-92626

SP

60aa

87L**

92979-94778

Unknown protein, SP

598aa

88R

94811-95263

Evrl-air-augmenter of liver regeneration

149aa

89R

95331-96497

SP

388aa

90R

96590-97981

Major capsid protein

462aa

91R

98105-99292

Immediate-early protein ICP-46

394aa

92R

99627-99872

SP

80aa

93L

100054-100221

SP

54aa

94L

100331-100798

Regina ranavirus P8.141 C-like protein, TM

154aa

95R

100891-101982

DNA repair protein RAD2, Xeroderma pigmentosum G N-region, Helix-hairpin-helix, Class 2 (Pol I) family

362aa

96R

102784-103455

Unknown protein, SP

222aa

97R

103538-103951

Myeloid cell leukemia protein, MCL-1, TM

136aa

98R

104649-104849

SP

65aa

  1. *Note: SP-surface protein, TM-transmembrane domain.
  2. ºLoss of original, predicted start codon in SSME; new start codon 27 bp downstream.
  3. §Loss of original, predicted stop codon in SSME; new stop codon 319 bp upstream.
  4. ¶39 bp deletion in original 50L ORF of SSME. Loss of ORF 50L’s original, predicted stop codon in SSME; ORFs 49L and 50L are now combined as 49/50L.
  5. #Deletion of 65L ORF and majority of 66L ORFs in SSME.
  6. **18 bp deletion in 87L ORF of SSME.