Figure 11

Effect of BVDV on Mo-DC cell surface marker expression. Differentiated Mo-DC were infected with either of four strains of BVDV, including ncp BVDV2a 1373, ncp BVDV2a 28508-5, or a homologous pair of ncp or cp BVDV1b viruses (ncp BVDV1b TGAN or cp BVDV1b TGAC) with an 6 MOI. The cells were stained for A) anti-MHCI; B) anti-MHCII or C) anti-CD86 antibody at 1, 6, 12, 24, 48, and 72 hour(s) post-infection. The mock-infected Mo-DC) at each time point was used as control (white legend). The mean fluorescent intensity (MFI) of expression was analyzed using flow cytometry. The each experiment was repeated three times. Asterisks (*) indicate significant difference from controls (P < 0.05).