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Figure 3 | Virology Journal

Figure 3

From: Genomic variation in macrophage-cultured European porcine reproductive and respiratory syndrome virus Olot/91 revealed using ultra-deep next generation sequencing

Figure 3

Variant validation using Sanger sequencing and realtime PCR. (A) Minor peaks representative of the quasispecies' genotypes could be seen under the highlighted major peaks from Olot/91. *The 3% low frequency variant can be further confirmed by the raw chromatogram (Additional file 2: Figure S2). (B) Variants from the main Olot/91 and its associated quasispecies resulted in different Ct curves.

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Virology Journal

ISSN: 1743-422X