Figure 3

Variant validation using Sanger sequencing and realtime PCR. (A) Minor peaks representative of the quasispecies' genotypes could be seen under the highlighted major peaks from Olot/91. *The 3% low frequency variant can be further confirmed by the raw chromatogram (Additional file 2: Figure S2). (B) Variants from the main Olot/91 and its associated quasispecies resulted in different Ct curves.