Comparison of all miRNAs assessed by RT-qPCR analysis of RNA isolated from plasma of JCPyV seronegative subjects (Ab−, n = 10) or JCPyV seropositive subjects (Ab+, n = 20). A. Volcano plot of pairwise comparisons. The volcano plot displays the relationship between fold-change and significance between the two groups, using a scatter plot view. The y-axis is the negative log10 of P values (a higher value indicates greater significance) and the x-axis is the difference in expression between two experimental groups as measured in log2 space. MiRNAs that were selected for further analysis are indicated in red on the plot. B. Mann-Whitney p-values, adjusted p-values and fold changes (fc) of JCPyV seronegative subjects (Ab−) vs. JCPyV seropositive subjects (Ab+) for miRNAs with p-value < 0.05. C. Relative expression levels of selected circulating miRNAs in subjects from each group. Data is presented relative to the mean of the entire population in log10 scale (n = 30).