Figure 2From: Detection and genetic characterization of Seoul Virus from commensal brown rats in FranceWorkflow for the preparation of lungs tissue samples for next generation sequencing. All samples were extracted using RNeasy mini kit (Qiagen) and treated by ScriptSeq complete golg kit (Epicentre) and submitted to Illumina sequencing. Comparison of viral enrichment methods: no enrichment procedure was performed for the S1 condition, a filtration step was included for S2 condition and a filtration step with nuclease digestion were combined for the condition S3.Back to article page