Figure 4
From: CRTC2 enhances HBV transcription and replication by inducing PGC1α expression
CRTC2 enhances HBV replication by inducing PGC1α expression. (A) PGC1α expression level in Huh-7 cells transfected with p-CRTC2 with or without FSK treatment. (B) HBV transcript amounts in Huh-7 cells transfected with 1.3× wtHBV plasmid and p-PGC1α. (C) Effect of PGC1α knock down on luciferase activity in Huh-7 cells transfected with pGL-3-ENII-luc and p-CRTC2 with FSK treatment. (D-F) Effect of PGC1α knock down on HBV mRNA level (D), HBV core protein expression (E), and HBV DNA replication intermediates (F) in Huh-7 cells transfected with 1.3× wtHBV plasmid and p-CRTC2 with FSK treatment. *p < 0.05; **p < 0.01.