High prevalence and diversity of species D adenoviruses (HAdV-D) in human populations of four Sub-Saharan countries

Table 3 Primers for specific and long-distance PCR

PCR	Gene	HAdV species	1^st-round primer (5′-3′)		2^nd-round primer (5′-3′)		3^rd-round primer (5′-3′)		Annealing temp. (C°)	Fragment size
PCR	Gene	HAdV species	Sense	Anti-sense	Sense	Anti-sense	Sense	Anti-sense	Annealing temp. (C°)	Fragment size
Generic PCR	Hexon	HAdV-D	AAGGCCGTCA	GTGCGGCTGG	ACAACTCGGGCTTCACCGGC	GGCTGGTGCA	None	None	63	322 bp
Generic PCR	Hexon	HAdV-D	CCCTGCCCTT	TGCACTCTGA	ACAACTCGGGCTTCACCGGC	CTCTGACCACG	None	None	63	322 bp
Long distance PCR	PVII-Hexon	HAdV-D	CGCCCAGCAA	GTGCGGCTGG	GGCAGGACTCGCAGACGAGC	GGTGGGCTCA	GCGCGGAAAC	GGGATGCGCC	60	4.8 kb
			TAACACCGGC	TGCACTCTGA		TCCATGGGGT	GTGTACTGGGT	ACATGACCCT

ISSN: 1743-422X