Table 2 The design groups for mouse experiment
No. of Sets | Vaccine | Adjuvant | Route | Number of mouse tested for (in each group) | |||||
|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | Â | Total in each group | Virus titration (3 and 5 dpi) | Survival | Lung Histopathology (3 and 5 dpi) | ELISPOT | Challenge viruses |
4 | 0.85% saline | Freund’s | i.m. | 11 | 3 for each time | 5 | - | - | H5N1 H1N1 H7N3 H9N2 |
4sM2 | Freund’s | 11 | 3 for each time | 5 | - | - | |||
1 | 0.85% saline | Freund’s | 17 | 3 for each time | 5 | 3 + 3 | - | H5N2 | |
4sM2 | Freund’s | 17 | 3 for each time | 5 | 3 + 3 | - | |||
1 | 0.85% saline | Freund’s | 8 | - | 5 (long lasting) | - | 3 | H5N2 | |
|  | 4sM2 | Freund’s |  | 8 | - | 5 (long lasting) | - | 3 |  |