Figure 4
Lung virus titers and histopathology. Virus titers in lungs tissues were determined by TCID50 in the MDCK cell line at 3 and 5 dpi with (A) A/EM/Korea/W149/06(H5N1), (B) A/PR/8/34(H1N1), (C) A/Aquatic bird/Korea/W81/2005(H5N2) respectively, (D) A/Aquaticbird/Korea/W44/2005(H7N3) and (E) A/Chicken/Korea/116/2004(H9N2) influenza subtypes. Bars denote mean ± standard deviations. Comparisons of groups were analyzed by Student’s t-test and ANOVA; the differences were statistically significant (P < 0.05). (F) Lungs of the vaccinated mice show clear alveoli without inflammatory cell infiltration as opposed to the lungs of control mice which revealed the features of severe pneumonitis. Tissues were observed by light microscope with 200× magnification.