Figure 3

Protective against divergent influenza virus subtypes. Five weeks-old female BALB/c mice were immunized twice with 30 μg of 4sM2 protein or 0.85% saline via i.m. Mice were infected intranasally with 10 times the 50% mouse lethal dose (MLD₅₀) of mouse-adapted influenza virus subtypes (A) A/EM/Korea/W149/06(H5N1), (B) A/PR/8/34(H1N1), (C) A/Aquatic bird/Korea/W81/2005(H5N2) respectively, (D) A/Aquatic bird/Korea/W44/2005(H7N3) and (E) A/Chicken/Korea/116/2004(H9N2) at 14 days after the final immunization Variations in body weight from the initial mouse body weight and percent survival were recorded until 13 dpi. The results are expressed as the mean ± standard deviations for the group.