Construction of a synthetic infectious cDNA clone of Grapevine Algerian latent virus (GALV-Nf) and its biological activity in Nicotiana benthamianaand grapevine plants

Table 1 Percent identity matrix of amino acid and nucleotide coat protein sequences from different GALV isolates

Isolates	GALV-Vv.1	GALV-Vv.2	Water doss	S. River	Gyp 2	Lim 3	Lim 4	GALV-Nf	Limo-08
GALV-Vv.1	―	99.73	99.20	93.35	96.28	99.94	93.88	95.48	95.74
GALV-Vv.2	99.91	―	99.47	93.62	96.28	98.2	93.88	95.74	96.01
Water Doss	99.13	99.73	―	93.09	95.74	99.73	93.35	95.21	95.48
S. River	85.08	85.5	85.29	―	94.41	92.82	91.49	93.88	94.15
Gyp 2	91.71	92.57	92.1	85.75	―	92.28	95.31	98.14	98.67
Lim 3	99.73	99.73	99.66	85.37	92.18	―	93.09	94.95	95.21
Lim 4	90.66	90.98	90.42	84.03	95.31	90.58	―	94.68	95.21
GALV-Nf	92.04	92.13	92.04	85.85	96.46	91.87	93.81	―	99.47
Limo-08	91.87	91.95	91.87	85.85	96.20	91.69	94.08	98.14	―

Deduced amino acid (above horizontal bars) and nucleotide (below horizontal bars) sequences from different isolates (from Algeria: GALV-Vv.1, GALV-Vv.2; from Germany: Water Doss, Schunter River (S. River), Gyp 2; from Netherlands: Lim 3, Lim 4 and from Japan: GALV-Nf, Limo-08) were aligned and a percent identity matrix was created. The lowest and the highest sequence identities between different isolates are shown in bold.

ISSN: 1743-422X