Table 2 Primers used in this study
Virus | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) | PCR product size (Target) | Purpose |
|---|---|---|---|---|
HPeV | CCYCTGGGSCCAAAAGSCA | GGTACCTYCWGGGCATCCTT | 145 bp (5′UTR) | Screening of HPeV |
AiV/SalV | CTGAGAMGRYGTTCCGCTGT | GACAATTAGCCCAGGSTCAGAT | 215 bp (5′UTR) | Screening of AiV/SalV |
HPeV | TCATGGGGTTCNCARATGGA | GATACCATAGTGYTTRTARAA | 774Â bp | Amplification of VP1 region |
AiV | TCTTCTCCTTCTACCGCTTG | GAGGTGTAGGGGATGGAGAA | 357Â bp | Amplification of VP1 region |
AiV | GCCAGTACAAGGACATGCGG | CGGTTGACGTTGACGCCAGG | 381Â bp | Amplification of 3CD region |
SalV | CCCCRTCAACTTCCAGCAAA | ACACGAACGATRGAGGTGCT | 482Â bp | Amplification of VP1 region |
SalV | GAGGGCACCGACCTGGATGC | TGGTTGATGAGAGAACCAAG | 439Â bp | Amplification of 3D region |