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Figure 2 | Virology Journal

Figure 2

From: CHD1 and CHD2 are positive regulators of HIV-1 gene expression

Figure 2

The mutagenic viral vector is integrated into the CHD2 gene in the mutant cell clones. (A) The TRE mutagenic viral vector is integrated into intron 2 of the hamster CHD2 gene. The DNA sequence around the integration site is expanded to show the exon2/intron2 boundary and the adjacent viral DNA (bold and underlined). (B) Quantitative RT-PCR was used to measure CHD2 mRNA levels relative to control β-actin mRNA levels in wild type and mutant cells. The ratios of CHD2 mRNA: β-actin mRNA were determined and compared to wild type CHO-K1 cells (defined as 100% Control). The data shown is the average mean of three independent experiments. Error bars indicate the standard deviation. The data was analyzed using an unpaired T-test, ***P value <0.0001.

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