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Figure 3 | Virology Journal

Figure 3

From: Avian paramyoxvirus-8 immunization reduces viral shedding after homologous APMV-8 challenge but fails to protect against Newcastle disease

Figure 3

Cross-reactivity of subtype specific immune sera with APMV-1 proteins. Gradient purified APMV-1 strain Clone 30 were lysed, and proteins were separated by SDS-PAGE, transferred on nitrocellulose and probed by IgY-specific POD conjugated sera as described previously[24]. Identity of proteins is indicated on the right, molecular weights of marker proteins (PEQLAB™ prestained protein marker IV) are indicated on the left. Lane 1–10 show results of hyperimmune sera raised against APMV-1 (1), APMV-2 (2), APMV-3 /P (3), APMV-3/T (4), APMV-4 (5), APMV-6 (6), APMV-7 (7), APMV-8 (8), APMV-9 (9) and PPMV-1 (10), respectively. Sera from naive chickens did not result in any band (data not shown).

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