Figure 1

Oropharyngeal APMV-8 shedding. Shedding of APMV-8 was tested at indicated days after infection of 3-weeks- (A) and 6-weeks- (B) old naive SPF-chickens or after reinfection (C) 3 weeks after priming. Boxplots represent results of APMV-8 specific RT-qPCR with infectivity equivalents (IEQ) derived from a standard curve prepared for each PCR-run. Testing for viral RNA was done by nucleocapsid protein (NP) specific RT-qPCR following a published protocol[11] with APMV-8 specific primer and probes. Numbers indicate the number of animals per group that tested positive for infectious virus on LMH cells as described previously[22]. Differences between time-points and groups were statistically tested by appropriate Wilcoxon-tests for paired and unpaired data respectively and labeled with specific symbols. Bonferroni correction was applied in case of multiple testing. The global significance level was 0.05. All calculations were performed using R software[23], version 2.13.0(2011-04-13).