Identification of an env-defective HIV-1 mutant capable of spontaneous reversion to a wild-type phenotype in certain T-cell lines

Table 5 Inhibition of G367R virus in MT2 cells by entry inhibitors

Entry inhibitor	Concentration	Continuous^a		Withdrawal
Entry inhibitor	Concentration	CPE^b	p24	CPE	p24
DS 003	2 nM	3/3^c	>600 ng/ml^d	3/3	>600 ng/ml^d
	20 nM	1/3	variable	3/3	>600 ng/ml
	200 nM	1/3	variable	3/3	>600 ng/ml
AMD3100	0.18 μM	3/3	>600 ng/ml	3/3	>600 ng/ml
	18 μM	1/3	variable	3/3	>600 ng/ml
	180 μM	1/3	variable	3/3	>600 ng/ml
Maraviroc	1.0 nM	3/3	>600 ng/ml	3/3	>600 ng/ml
	10 nM	3/3	>600 ng/ml	3/3	>600 ng/ml
	100 nM	3/3	>600 ng/ml	3/3	>600 ng/ml

MT2 cells were infected by pseudotyped G367R viruses at 4-fold dilutions and grown in the presence of entry inhibitors. The cultures were then split into 2 wells at day 7 with only one of them being maintained in the presence of inhibitors.
^aContinuous represents the maintainance of the entry inhibitors in the cultures. Withdrawal represents drug withdrawal after 7 days of infection.
^bCPE = cytopathic effect.
^cNumbers of samples in which CPE was observed over 5 weeks after infection.
^devaluated during 28 days following infection. p24 values of CPE negative samples were lower than listed.

ISSN: 1743-422X