Identification of an env-defective HIV-1 mutant capable of spontaneous reversion to a wild-type phenotype in certain T-cell lines

Table 2 Dose dependent reversion of G367R + VSV-G in MT2 and MT4

Cells and numbers		Viruses used and time of appearance of CPE
		Virus dilution^a	G367R		G367R + VSV-G
			CPE^b	p24	CPE	p24
MT2	10⁴	4×	(0/24)^c	0	14d (24/24)	>600 ng/ml^d
	500	4×			14d (16/16)	>600 ng/ml
	500	12×			21d (14/16)	>600 ng/ml
	100	36×			28d (15/16)	>600 ng/ml
MT4	10⁵	4×	(0/3)	0	14d (2/3)	>600 ng/ml
	5000	12×			(0/3)	<50 ng/ml
	5000	36×			(0/3)	<50 ng/ml

MT2 or MT4 cells were incubated with viruses at different dilutions for 3 hours, washed and resuspended in fresh media, and split into wells at appropriate concentrations.
^avirus dilution used for infection.
^bCPE = cytopathic effect.
^cnumber (d = day) represents the time of first appearance of CPE in the last sample analyzed over 28 days. Numbers (in parentheses) designate the numbers of samples in which CPE was observed.
^devaluated during 28 days after infection. p24 values of CPE negative samples were lower than listed.

ISSN: 1743-422X