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Table 2 Dose dependent reversion of G367R + VSV-G in MT2 and MT4

From: Identification of an env-defective HIV-1 mutant capable of spontaneous reversion to a wild-type phenotype in certain T-cell lines

Cells and numbers Viruses used and time of appearance of CPE
Virus dilutiona G367R G367R + VSV-G
  CPEb p24 CPE p24
MT2 104 4× (0/24)c 0 14d (24/24) >600 ng/mld
500 4×    14d (16/16) >600 ng/ml
500 12×    21d (14/16) >600 ng/ml
100 36×    28d (15/16) >600 ng/ml
MT4 105 4× (0/3) 0 14d (2/3) >600 ng/ml
5000 12×    (0/3) <50 ng/ml
5000 36×    (0/3) <50 ng/ml
  1. MT2 or MT4 cells were incubated with viruses at different dilutions for 3 hours, washed and resuspended in fresh media, and split into wells at appropriate concentrations.
  2. avirus dilution used for infection.
  3. bCPE = cytopathic effect.
  4. cnumber (d = day) represents the time of first appearance of CPE in the last sample analyzed over 28 days. Numbers (in parentheses) designate the numbers of samples in which CPE was observed.
  5. devaluated during 28 days after infection. p24 values of CPE negative samples were lower than listed.
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