|
Cells and numbers
|
Viruses used and time of appearance of CPE
|
|---|
|
Virus dilutiona
|
G367R
|
G367R + VSV-G
|
|---|
| |
CPEb
|
p24
|
CPE
|
p24
|
|---|
|
MT2
|
104
|
4×
|
(0/24)c
|
0
|
14d (24/24)
|
>600 ng/mld
|
|
500
|
4×
| | |
14d (16/16)
|
>600 ng/ml
|
|
500
|
12×
| | |
21d (14/16)
|
>600 ng/ml
|
|
100
|
36×
| | |
28d (15/16)
|
>600 ng/ml
|
|
MT4
|
105
|
4×
|
(0/3)
|
0
|
14d (2/3)
|
>600 ng/ml
|
|
5000
|
12×
| | |
(0/3)
|
<50 ng/ml
|
|
5000
|
36×
| | |
(0/3)
|
<50 ng/ml
|
- MT2 or MT4 cells were incubated with viruses at different dilutions for 3 hours, washed and resuspended in fresh media, and split into wells at appropriate concentrations.
- avirus dilution used for infection.
- bCPE = cytopathic effect.
- cnumber (d = day) represents the time of first appearance of CPE in the last sample analyzed over 28 days. Numbers (in parentheses) designate the numbers of samples in which CPE was observed.
- devaluated during 28 days after infection. p24 values of CPE negative samples were lower than listed.
