Identification of an env-defective HIV-1 mutant capable of spontaneous reversion to a wild-type phenotype in certain T-cell lines

Table 1 G367R + VSV-G reversion in different cell types

Cells and numbers		Viruses used and time of appearance of CPE
		G367R		G367R + VSV-G
		CPE^a	p24	CPE	p24
MT2	10⁵	No^b	0	8-12d (3/3)^c	>600 ng/ml^d
	5×10⁴			9-15d (6/6)	>600 ng/ml
	10⁴			11-21d (24/24)	>600 ng/ml
	10²			21d (16/16)	>600 ng/ml
MT4	10⁵	No	0	14d (2/3)	>600 ng/ml
SupT1	10⁴	No	0	21d (6/6)	>600 ng/ml
SupT1	5×10²			35d (24/24)	>600 ng/ml
PM1	10⁴	No	0	(0/6)	0

Cells were incubated with viruses at 4-fold dilution for 3 hours, washed and resuspended in fresh media, and split into wells at appropriate concentrations.
^aCPE = cytopathic effect.
^bNo = CPE not observed over 28 days.
^cnumber (d = day) represents the time of first appearance of CPE in the last sample analyzed over 28 days. Numbers (in parentheses) designate numbers of wells in which CPE was observed.
^devaluated during 28 days after infection. p24 values of CPE negative samples were lower than listed.

ISSN: 1743-422X