Lower levels of activating phosphorylation of Akt, p70S6K, and eIF4B in cells expressing CyPrPEGFP. Targeted tertiary analyses of mTOR signaling in N2a cells expressing CyPrPEGFP for 12, 24, or 48 h. The normalized absolute phosphorylation levels of 10 protein kinases or substrates in each of the three biological repeats are shown by the color bars. The phosphorylation sites evaluated are indicated above each protein. Proteins indicated by dashed lines were not analyzed. Due to limited sample, the levels of p-Akt at 12 h were measured only in two of the three biological repeats. The color bars for S6 indicate the normalized phosphorylation levels of S235/236 (top) and S240/244 (bottom).