Identification of the mTOR signaling pathway as potentially dysregulated in cells expressing CyPrPEGFP. Hierarchical clustering of 76 protein kinases using the normalized and log2 transformed densitometric data from primary multiplex Western blots. Red, higher expression levels; green, lower expression levels. Each category encompasses changes in expression levels of 18% (0.23 in log2 scale), 3 times the 6% linear changes detected by the technique. Grey boxes indicate clusters of protein kinases most differentially expressed in cells expressing CyPrPEGFP. The clusters ( i ) and ( ii ) consist of PKCα, p70S6K, Akt1, and AMPKα1 involved in mTOR signaling. The protein kinases highlighted in Figure 4, p70S6K and PKACβ, are indicated by (●).