Figure 1

Multiplex Western blots detect 122 selected protein kinases in only 1.2 mg of mouse brain. A single-well gel was loaded with 1.2 mg of mouse brain homogenate, and the proteins were resolved and transferred. A multi-screen apparatus isolated 16 individual lanes within the area of homogeneously resolved protein and probed with optimized antibodies specific for 122 selected protein kinases. Molecular weights in kDa are indicated on the left. Signal from secondary antibody labeled with Alexa Fluor 680 (red bands) and IRDye 800 (green bands) was detected using a LI-COR Odyssey infrared imaging system. Yellow bands, red-labeled goat anti-mouse secondary antibody detected by green-labeled anti-goat secondary antibody. Due to the wide range in expression levels, no single exposure of the blot can show all the bands. The bands that are visible in each lane at the exposure shown are listed (from top to bottom) in the main text.