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Table 4 Primer sets used for PCR amplification of F and HN genes of Ugandan NDV isolates

From: High pathogenicity and low genetic evolution of avian paramyxovirus type I (Newcastle disease virus) isolated from live bird markets in Uganda

Gene

Forward primer (5′ to 3′)

Reverse primer (5′ to 3′)

PCR product (bp)

F

NDVF1F1: GCAAGATGGGCYCCAAACC

NDVF1R2: CATCTTCCCAACTGCCACT

550

 

NDVF2F2: GACCACTTTACTCACTCCTC

NDVF2R1: GTAGGTGGCACGCATATTATT

642

 

NDVF3F2: CGACTCACAGACTCAACTC

NDVF3R2: TATARGTAATRAGRGCRGATG

685

 

NDVF4F2: GCAAGATRACAACATGTAGRTG

NDVF4R2: CTTGGCTAACYGCRCGGTCCAT

709

HN

NDVHN1F2: GGCTTCMCAACATCCGTTCTAC

NDVHN1R2: GAATGYGAGTGATCTCTGCA

652

 

NDVHN2F2: CATGAGYRCTACCCAYTACTG

NDVHN2R2: GATAGATAAGATGGCYTGCTG

591

 

NDVHN3F2: GGGTGGCAAAYTACCCAGGAG

NDVHN3R2: GTATTGGATATTTCRGCAATGC

768

 

NDVHN4F2: GCATACACGACATCGACATG

NDVHN4R2: CGGTARCCCAGTYAATTTCCA

513

  1. 1modified from [16].
  2. 2modified from [32].
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Virology Journal

ISSN: 1743-422X