Influences of ETV combined with CMK on the HBV replication and HBeAg secretion. HepG2.2.15 cells were regularly cultivated and treated with or without CMK (20 μmol/L) and/or ETV (30 nmol/L). Intracellular core-associated HBV DNA was detected using Southern blot analysis. Relaxed circular (RC) HBV DNA was the HBV genome, and single-stranded linear (SS) HBV DNA was the replication intermediates. Virion-related HBV DNA in culture medium was quantified using real-time fluorescent PCR. HBeAg and HBsAg were detected using enzyme-linked immunosorbent assay. A: ETV abrogated the HBV replication-enhancing effect of CMK. B: ETV combined with CMK reduced HBV replication and HBeAg secretion simultaneously. **P < 0.01.